Journal: European Journal of Medical Research
Article Title: Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model
doi: 10.1186/s40001-025-02344-6
Figure Lengend Snippet: Effects of OM-MSCs Nrf2 on the levels of relevant cytokines in PC12 cells. A Flow cytometry was used to characterize CD45, CD34, CD29, CD90, CD73, and CD105 of OM-MSCs. B RT-qPCR-based to assess transfection efficiency after overexpression of Nrf2 plasmid. OM-MSCs Nrf2 : NRF2 overexpression group. OM-MSCs (ctrl): Empty vector transfection group. OM-MSCs: Untransfected OM-MSCs. C Morphology of both OM-MSCs (ctrl) and OM-MSCs Nrf2 under the optical microscope. Scale bar: 100 μm. D The changes in cytokine concentrations in PC12 cells measured by ELISA, including IL4, IL10, IL13, VEGF-A, and TGF-β. Results of independent triplicates were expressed as mean ± standard deviation and the statistical difference between two groups was marked with asterisks (“ns” indicates no significant difference, * p < 0.05, and ** p < 0.01). OM-MSCs olfactory mucosa-mesenchymal stem cells, Nrf2 : Nuclear Factor Erythroid-Derived 2-Like 2, IL interleukin, VEGF-A vascular endothelial growth factor-A, TGF-β transforming growth factor beta
Article Snippet: The isolated OM-MSCs were stained with the following antibodies: phycoerythrin (PE)-labeled anti-CD45 antibody (#202207, 1:500, BioLegend, Inc., San Diego, CA, USA), PE/Cy5.5-labeled anti-CD34 antibody (#NBP2-33076PECY55, 1:500, Novus Biologicals, Centennial, CO, USA), fluorescein isothiocyanate (FITC)-labeled anti-CD29 antibody (#sc-9970 FITC, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), Alexa Fluor® 647-labeled anti-CD90 antibody (#ab317534, 1:500, Abcam, Cambridge, UK), Alexa Fluor® 488-labeled anti-CD73 antibody (#NBP2-71214AF488, 1:500, Novus Biologicals, USA), and allophycocyanin (APC)-labeled anti-CD105 antibody (#NB500-452APC, 1:500, Novus Biologicals, USA) [ ].
Techniques: Flow Cytometry, Quantitative RT-PCR, Transfection, Over Expression, Plasmid Preparation, Microscopy, Enzyme-linked Immunosorbent Assay, Standard Deviation, Derivative Assay
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